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文献和实验centrifuge bottle, centrifuge at 10,000 rpm for 30 minutes at 4deg C in the RC5-B using the GSA rotor. For cesium chloride-gradient purification: 7a. Pool the cleared supernatants into to a clean beaker, add one-fourth volume of 50% PEG/0.5 M NaCl
Construction and Characterization of Adenovirus Vectors
), 1.25 g/mL and 1.35 g/mL, in dialysis buffer Prepare each gradient solution separately. Sterilize by filtration through a 0.2-µm filter. Chloroform:isoamyl alcohol (24:1) Citric saline (2X) For 1X citric saline, dilute the 2X
ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
) percoll A:buffer A. Percoll A is made as follows: to 34.23 g sucrose add 1.0 ml of 1 M Tris-HCl (pH 7.2), 0.5 ml of 1 M MgCl2 , 34ul 2-mercaptoethanol, and Percoll to a final volume of 100 ml. Centrifuge in a JS-13 rotor at 2000 rpm after 5 min. increase
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