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文献和实验°C for X min(1min/kb) Final extension 72°C for 7 min Final soak 4-10°C 6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling
6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling program. 7) Simplified hot start: Start the PCR program. Once the thermal cycler has reached 94°C
【共享】经验:The RNeasy Mini Kit from Qiagen
The RNeasy Mini Kit from Qiagen is designed for RNA extraction, although the basic format is similar to other Qiagen products designed to purify nucleic acids: ion exchange columns specifically bind RNA which is purified by wash steps
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