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文献和实验Overview This is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are currently several BioBrick assembly standardsthat all involve assembly by restriction enzyme
which have added adapter sequences. Thus PCR followed by Gibson Assembly allows you to join any two blunt ended pieces of DNA. Up to 10-20 different pieces of DNA can be neatly spliced together in one reaction using these techniques.
In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
-free systems. Nucleotide analog interference mapping (NAIM) can report detailed atomic information about requirements for ribonu-cleoprotein particle assembly and function in living cells, providing a method to study complexes in a cellular context
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