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文献和实验. Worms dyed late in the afternoon can be stored at 15°C overnight (o/n) and viewed the next morning. Microfuge tube Label 1.7 ml microfuge tube with strain name. Squirt ~1 ml M9 onto plate with a pasteur pipet. Rinse plate with M9
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
procedure1. Remove the growth medium from the cells to be assayed. Rinse the cells twice in PBS buffer, being careful not to dislodge any of the cells.2. Add a minimal volume of 1 x CCLR to cover the cells. (250 µl for a 60 mm dish) and incubate for a short
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