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文献和实验are precipitated with sodium acetate, and the lysate is cleared first by filtration of precipitate through cheesecloth and then by centrifugation. The DNA-containing supernatant is transferred to a new tube, and the plasmid or cosmid DNA is precipitated
. Worms dyed late in the afternoon can be stored at 15°C overnight (o/n) and viewed the next morning. Microfuge tube Label 1.7 ml microfuge tube with strain name. Squirt ~1 ml M9 onto plate with a pasteur pipet. Rinse plate with M9
DNA Extraction from Frozen Tissue Sections
at room temp. 10.Remove aqueous layer into a new tube and repeat the PCI extraction (steps 8-10). 11.Aliquot the aqueous phase into as few 1.5 ml epindorftubes as possible. Maximum volume per tube is 350 μls. Add 1/2 volume of 7.5 M ammonium acetate
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