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文献和实验of interest from PVDF membranes with a scalpel or cold punch, on a minimum of PVDF. Place each spot into a 700 µl clear glass autosampler vial. If possible, use vials that can later be used directly on your AA analysis autosampler. If spots are very feint
Adjust the wavelength to that specificied for the procedure you are using. Be sure the cover is closed on the cuvette holder and use the left knob on the front panel to adjust the dark current such that the meter is reading
Be sure the cover is closed on the cuvette holder and use the left knob on the front panel to adjust the dark current such that the meter is reading 0 transmittance. At this point, you are simply adjusting the internal electronics of the instrument
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