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文献和实验screws, 1 pair of tweezers, one 75 x 25 x 1 mm glass slide (or other desired cell surface material), and 2 surgical towels. 6) Place sterile towels into laminar flow hood. Place the flow circuit, flow chamber, 4 x 4-way stopcocks, 1 x 1-way
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
on a glass slide ~7-10 mm apart and cover with a 18x18 or 22x22 mm coverslip. This results in a ~12-15 µl flow cell. Solutions are pipeted on one side and sucked out the other side by capillary action using Whatman #1 filter paper or a Kimwipe. Washes in flow
microscope. This will allow observation of the cells without opening or disturbing the growth. 16. Make cell density determinations at 10 X magnification using a square ocular grid, as explained in Chapter One for the determination of area. 17. Plot
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