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文献和实验Sample preparation (analytical gels)
morpholinopropane sulfonate (MOPS), plus thiamine at 37oC. Growth was stopped in the late exponential phase at an OD of 1 at 600 nm. Five hundred ml of culture medium was centrifuged for 30 min at 3000 rpm at 4\xa1 C and the pellet was washed 4 times for 10 min
Purification of Plasmid from 50 ml-culture
2. Spin the bacterial culture at 6,000 rpm for 10 min at 4 C. Discard the supernatant. 3. (0ptional) Suspend the cell in about 20 ml of deionized water. Spin again. Discard the supernatant. Remove all of the supernatant fluid using pipetman.
Sample preparation (analytical gels)
in cold rinse buffer (glutamine-free RPMI 1640 medium containing 5 % fetal calf serum,0.2 mM phenylmethylsulfonyl fluoridem,1mM EDTA,and antibiotics: oxacillin 25 µg/ml,gentamycin 50 µg/ml,penicillin 100 U/ml,streptomycin 100 µg/ml,amphotericin B 0.25 µg
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