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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
3) Puromycin, G418, and Ganciclovir Kill Curves A. Perform a kill curve for ganciclovir with cells stably expressing the Target ID Library and with puromycin or G418 for wild type cells. B. Plate 1.6 x 104 cells
from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
Phycoerythrin conjugation protocol
. I used 117 x 1 cm. You can't really get away with anything smaller. Use a long thin one rather than a short fat one, since the latter did not work for me. Separation of the conjugate from the free reagents is tricky. *
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