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文献和实验Phosphoamino acid analysis:Mark Kamps's method
will work! Keep the membrane wet and wrap in Saran wrap. Add radioactive markings for orientation of filmlater Expose to film. 2.Cut out band of interest,re-wet in methanol,rinse well in water and place in a screw-cap tube containing 150 µl 5.7 NHCl
Phosphoamino acid analysis:Mark Kamps method
in a screw-cap tube containing 150 µl 5.7 N HCl or enough to submerge your piece of membrane. 3. Incubate in 110°C oven for 60 min. 4. Microfuge sample full speed for at least 1 min. 5. Transfer supernatant to new tube and lyophilize
Aluminum Alloy N/A 6061 Cell-Culture Dishes (4-well, rectangular) Thermo Scientific
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