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文献和实验Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
found in clinical samples (variations in cellular functions, lineages, and clonotypic breadth) requires approaches for monitoring immune responses with single-cell resolution. In this chapter, we present an engineering approach for integrated single-cell
Recording Single-Channel Currents Using Smart Patch-Clamp Technique
-clamp pipette to record ion channel signals from specific spots on the cell surface as determined by SICM. In this chapter we focus on recording single channel L-type calcium channel currents from T-tubules of adult rat
Single-Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli
podocytes in the freshly isolated decapsulated intact glomeruli. Here we describe the preparation of the rat glomeruli for patch-clamping, focusing on special conditions required for single-channel analysis of TRPC channels. Several tricks useful for cell
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