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文献和实验Technologies) 100% ethanol 2X loading dye (95% formamide, 20 mM EDTA, 0.05% bromophenol blue, 0.05% xylene cyanol) 2. Methods (Under sterile, RNase free conditions) Note: The protocol was utilized to screen for tumor-specific alterations
variations) prepare mastermix for all PCR reactions which contain the same T12 MN primer, aliquot 18 µl into each tube and then add the arbitrary primer (= decamer) mix for each 20 µl PCR reaction: 9.2 µl H2 O, 2 µl 10 x PCR reaction buffer
Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
). Transformation by vacuum infiltration Six mg of WS seeds (about 300 seeds) were sown on compost in 40x30 cm sowing flats . The germination was synchronized by cold treatment for 48 h at 40C, and the flats were placed in greenhouse (16 hours day
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