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文献和实验Live imaging with Drosophila tissue culture cells
with most Drosophila tissue culture cells as long as they are semi-adherent. I tested a variety of different Drosophila Schneider S2 cells for their growth behavior and found that most of them had some difficulties undergoing and completing cytokinesis, producing
of embryos, by delicate resuspension using wide aperture tips, onto a clean slide and cover with a 22x22 mm coverslip. Seal the edges with transparent nail polish. Slides can be stored for a few weeks at 4°C in the dark. In our experience, the DAPI stain
concentration of 10%. For example, for a 100μl sample, the formula is 100μl + 0.1X = X (where 0.1 represents the 10% final TCA conc), or X=11.1 μl. 5) Mix well by inverting 5-6x, then place on ice for > 30min. This step can go overnight, as long as the samples
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