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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
), 10 µl per liter of a 1 mg/ml stock in DMSO. By adding the hormone just before use, you can keep infiltration media as a stock for at least one week prior to infiltration. - we recommend to add 0.01% silwet to the infiltration media to increase
Standard Operating Procedures for T1-Phage Testing Assay
I. Introduction: This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard LB agar. If phage is present in a clone streaked or stamped onto the top agarose
Bromodeoxyuridine Staining of Mammary Tissue
from a post-pubertal mouse.) II. BrdU Detection A. Perfusion fixed, paraffin sections: 1. Deparaffinize sections using standard xylene/alcohol procedures. 2. Treat sections with 0.1% Trypsin in 0.1M TBS (pH7.5) + 0.1% CaCl2 for 20 minutes at 37
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