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文献和实验Use of Internal and External Standards or Reference RNAs for
and authenticating this control are critical to successful relative quantitation. In the paragraphs below, we describe commonly used internal controls and how these are authenticated. Internal Standard RNA Probes Inconsistencies in RNA isolation
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
Real-Time qRT-PCR standard curves... efficiency is too h
Greetings, In my Real-Time qRT-PCR experiments, I employ the standard curve method for quantification of gene expression. However, standard curves seem to be a huge hit-or-miss procedure for me, even with genes that are well-established
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