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文献和实验to the animal and provides biologically meaningful samples. Only personnel with appropriate AHR training may handle live animals. Isoflourane inhalation: This should be given in sufficient dose to cause complete loss of breathing and pulse in the mouse. Apply
a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
upon the amount of tissue used. 10. Count the cells using a hemacytometer and add the appropriate number to a nontreated polystyrene dish containing NEP complete medium. Cell density should be 1 x 106 cells per 6-cm plate, or 2 x 106 cells per 10-cm
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