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文献和实验9M Urea,2.5mM EDTA,2.5mM EGTA,1% DTE,4% CHAPS make 10ml and aliquot 10x1ml,freeze at -70℃ Lysate preparation wash the cells 2x with PBS wash the cells 1x with 10mM Tris,250mM Sucrose lyse the cells with 100 – 350μl of urea lysis buffer
3% TREVIGEL PROTOCOL for 3 bp resolution
can be obtained by running the gel in 1X TAE at 5 volts/cm for 2 hours with a 40 cm gel. For SSR marker screening in 20 x 40 cm gels: For 400 ml of gel solution: At room temperature, stir 350 ml 1X TAE buffer in a 1 Liter screw cap bottle
or pencil to correspond with the number of each respective plate. 25 G needle and small drop of India ink on piece of parafilm. Tray with base solution . Whatman 3MM filter paper or gel blot paper to blot filters dry after removal from base
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