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文献和实验ensures a low background level of nonspecific probe adherence to the membrane and, second, an extended period of filter prehybridization is not required. The inclusion of a large amount of SDS does, however, necessitate that the nucleic acids
Filter Binding Assays for TopoisomeraseDNA Complexes
and DNA are not retained. Experimental data can thus be obtained from analysis of the nucleic acids retained in the filter, or found in filtrates and washes. Because protein-adsorbing materials can differ in their efficiency, capacity, and flow
Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization
, and the data reproducibility is high. However, there may be cases in which automated systems are not available or are unnecessary, for example, if the YAC library is small. In this chapter, we describe a simple method to process YAC libraries manually
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