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上海希言科学仪器有限公司
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文献和实验l of 4mM acetyl CoA 2.0 µl 750 µM HCl (10X) 16.25 µl H2 O 1.0 µl 3H acetyl-CoA (Times number of samples) 15. Add 20 µl of mix into each tube (reaction & blank). 16. Incubate for 2 hrs @ 37C. 17. Add 500 µl of toluene
Single tube confirmation PCR protocol
results were obtained when 1 µl, 5 µl or 10 µl of the Zymo treated cells were used as template. 3. PCR reactions - The lyophilized confirmation primers (~5-10 nmoles of each oligonuleotide) should be resuspended in 750 µl of TE (final
Mesoplasma florum:Transposome construction
Wash once with 500 μl buffer PB Wash 2x with 750μl buffer PE, spin at high speed for 5 minutes after emptying. Elute with 2x 50 μl buffer EB into a clean tube. Add 10 μl NEB buffer 2 Add 37 μl water Add 3 μl PvuII, mix
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