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文献和实验Optimal arbitrary primer length for Differential Display（图）
as more than one band in each lane) should be kept to a minimum.As a result, arbitrary primer length is increased from the sub-optimal value of 9-10 bases to 13 bases. The 13mers fulfill the above criteria for being optimal arbitrary primers (Liang et al., 1994, NAR. 22
UV Absorbance (280 nm) Protein Determination
) in the buffer may also help to prevent these losses. The value obtained will depend on the path length of the cuvet. If not 1 cm, it must be adjusted by the appropriate factor. The Beer-Lambert law states that: A (Absorbance) = Ê.c.l where Ê
no more than 1-2 days in length, and during which there is little or no increase in cell number. During this time, the cells are "conditioning" the media, undergoing internal cytoskeletal and enzyme changes and adjusting to the new media.This is followed by a Log
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