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文献和实验In Vivo Luciferin Imaging Procedure
is plumbed to the anesthesia manifold located inside the imaging chamber. After the mice are fully anesthetized, they are transferred from the box to the nose cones attached to the manifold in the imaging chamber, the door is closed, and the “Acquire” button
denaturation of DNA will result in the inefficient use of the template in the first amplification cycle and, consequently, poor yield of PCR product. The annealing temperature may be estimated as 5°C lower than the melting temperature of the primer-template
before experiement) take out filtered gel aliquot and fluorescent dye from fridge next door 30min ahead of time (1 gel aliquot tube enough for 2 chips) take out ladder from fridge upstairs vortex dye 10s and spin down mix one tube gel
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