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文献和实验is achieved. Insert the needle's shaft into a 5 m l capillary pipette and seal it in by immersing the wire-containing portion of the capillary in melted paraffin until it is heated through. In a flame, quickly burn off the paraffin adhering to the exposed
Collection of Peritoneal Cells
Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important. Prepare a Pasteur pipette with rubber pipetter. Euthanize by CO2 inhalation. Ask
Collection of Peritoneal Cells
the smallest possible hole. Remove the needle. The peritoneum should self-seal. Hold the mouse in your hand and shake (image) it vigorously about ten-fifteen times. Put it back on the table. Grab the sternum and some peritoneum with a tweezer
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