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文献和实验Sequencing Double-Stranded DNA Using the Sequenase Version 2.0 Kit
实验步骤 1. Prepare the DNA for sequencing as per the protocols, "Purification and Concentration of Aqueous DNA Solutions" and "Alkali Denaturation of Supercoiled Plasmid DNA". 2. Warm a heating block up to 65 degrees
Transfer up to 700 μL of the sample (including any remaining precipitate) to the spin cartridge (with the collection tube). 4) Centrifuge at 12,000 × g for 15 seconds at room temperature. Discard the flow-through, and reinsert
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
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