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文献和实验. Although these cells are routinely split 1:2, anyone handling these cultures is requested to assess the viability of the population. 0.5 ml of the cell suspension was added to a tube containing an equal volume of trypan blue, the tube was aqitated
. Although these cells are routinely split 1:2, anyone handling these cultures is requested to assess the viability of the population. 0.5 ml of the cell suspension was added to a tube containing an equal volume of trypan blue, the tube was aqitated
from the cultures. Place the sample in a separate test tube (it need not be sterile). 2. Dilute 4 parts of stock Trypan Blue with 1 part of 5X saline and add 0.1 ml of the diluted dye to your sample. Mix gently. 3. Set up a hemocytometer and cover slip
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