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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
-based methods,also work very well. We found that the Qiagen kit designedfor purification of DNA from agarose gel is relatively convenient,giving rise to high yield and clean PCR products. 4. For cloning of large PCR products, TOPO-TA-XL worksbetter
【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
Methods in Molecular Biology vol.687 Park D.J. (ed.) PCR Protocols Chapter 5 PART II :Cloning and Sequencing CODEHOP PCR and CODEHOP PCR Primer Design Jeannette P. Staheli, Richard Boyce, Dina Kovarik,and Timothy M. Rose
Bisulfite sequencing of very small samples
in the middle or at the 5’ end of the primer and it does not provide a good template for Taq pol (which could inactivate this primer pair). Keep the PCR product under 600 bp (ideally 300-350 bp). Day 1 (if your starting material is DNA go to day
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