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文献和实验Natural History Museum Protocol for EST sequencing from lamb
at the correct pH. Add 10µl of each deoxyribonucleotide solution to 760µl water to give 800µl of 1.25mM dNTP mix, aliquot and store at -20℃. PCR primers: We use T3 ( 5¹-AATTAACCCTCACTAAAGGG -3¹) and T7 (5¹-GTAATACGACTCACTATAGGGC-3¹) primers at 20pm/µl.(4) Run
Whole genome amplification protocol for ChIP-chip
to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
of 1.25mM dNTP mix, aliquot and store at -20oC. PCR primers: We use T3 ( 5¹-AATTAACCCTCACTAAAGGG -3¹) and T7 (5¹-GTAATACGACTCACTATAGGGC-3¹) primers at 20pm/µl. (4) Run out 8µl of the PCR reaction on a 1% agarose gel to check
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