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文献和实验. INTRODUCTION This protocol describes the analysis of a proteome using multidimensional protein identification technology (MuDPIT), which couples 2D-LC to MS/MS, to resolve and identify peptides from complex mixtures. In this method, a pulled
Determining In Vivo Phosphorylation Sites Using Mass Spectrometry
phosphorylation sites from whole?cell and tissue extracts. For the first, endogenous or epitope?tagged proteins are typically immunopurified from cell lysates, purified via gel electrophoresis or precipitation, and enzymatically digested into peptides. Samples
Identification of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry
Figure 5.6.1 Integrated two‐dimensional liquid chromatography. Peptides are separated in the first dimension by strong cation exchange (SCX) followed by reversed‐phase (RP) separation and elution into the mass spectrometer. Triangles, circles
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