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文献和实验Sucrose Fractionation of Castor Bean
that the stepped gradients for both sections are exactly the same weight. Add grinding media to balance, where appropriate. Have the instructor check the balance before proceeding. 11.Centrifuge the tubes at 4° C for 4 hours at 25,000 RPM in a Beckman SW27 rotor
. Fractions are assayed by spindown and double IF with 5051 serum and anti-tubulin and the pooled fractions are frozen in liq N2. What you need: Equipment: refractometer Sorvall with cold HB-4 Ultra with cold SW
and the pooled fractions are frozen in liq N2. What you need: Equipment: refractometer Sorvall with cold HB-4 Ultra with cold SW27 Some sort of gradient fractionator with a fraction collector 16 30 ml corex
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