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文献和实验Transfer of Eukaryote Suspension Cultures
of a stock bottle of tissue culture media. 6 Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. Open the top of the suspension
Subculture of Suspension Cell Lines
are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover the monolayer with trypsin. Decant the excess trypsin. Return flask to the incubator and leave
Handling Fresh Hepatocytes in Suspension
实验试剂 Dexamethasone, 10 mM in DMSO solution, 1 x 250 μL (supplied in excess) Maintenance Cocktail solution, 1 x 20 mL, containing: 2.5 mL penicillin-streptomycin, 10,000 U/mL 5.0 mL ITS (insulin
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