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文献和实验Crystallization of Kinesin Family Motor Proteins
parameters.Crystals of monomeric rat Kinesin-1Table 1: Crystallization conditions for kinesin motor protein constructsConstructMethodConditionsReferencesHuman Kinesin-1 hK349Sitting drop 4°C5 mg/ml protein in 50 mM Na-acetate, pH 4.6, 75 mM KCl, 3.5% (w/v
(Spectrum) 200‐ to 300‐mesh silica gel (Natland, http://www.natland.com/) Ethyl acetate (EtOAc), HPLC grade (Fisher Scientific
V294.PART II,Chapter 5 细胞粘附实验(英文版)
(8). With minor modifications,it can be used to analyze static adhesion of almost any cell types. 1. Coat wells of 96-well ELISA plates with 10 μg/mL fibronectin in PBS at 37°Cfor at least 1 h (see Note 1). 2. Incubate each well with 200 μL of heat-denatured
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