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上海希言科学仪器有限公司
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文献和实验Homogenizer, RNase-free syringe (1 mL) with 18–21-gauge needle or, Rotor-stator homogenizer Microcentrifuge capable of centrifuging 12,000 × g 15 mL RNase-free tubes (for samples with >107 cells) 实验步骤 1.
Fastfilter Plasmid Midi Kit Spin Protocol
into a 15 ml collection tube, provided. Add 2 ml of Buffer GPS to the column and Lit it sit at room temperature for 3-10 min. Spin in a swinging bucket rotor at 3,000-5,000 x g for 5 minutes at room temperature. Discard the eluate and assembled the column
Fastfilter Plasmid Maxi Kit Spin Protocol
Column into a 50 ml collection tube, provided. Add 5 ml of Buffer GPS to the column and Lit it sit at room temperature for 3-10 min. Spin in a swinging bucket rotor at 3,000-5,000 x g for 5 minutes at room temperature. Discard the eluate and assemble
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