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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times
, and vacuum filter it. Repeat the 1x column buffer treatment cycle, adjusting the pH of the slurry each time to 6.6 with 10M NaOH, until the pH of the slurry is 6.6 without adjustment after resuspension and mixing. Allow the resin to settle for 20 min
E.Z.N.A.® Dried Blood DNA Spin Protocol
Buffer, and incubate at 65℃ for 1 hour, while vortexing every 20 min for proper mixing. 3. Add 25μl of OB Protease stock solution and mix well. Incubate for 30 min at 65℃ with occasional mixing. Note: Step 6 can be performed
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