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文献和实验Phosphoamino acid analysis:Mark Kamps's method
or enough to submerge your piece of membrane. 3.Incubate in 110℃ oven for 60 min. 4.Microfuge sample full speed for at least 1 min. 5.Transfer supernatant to new tube and lyophilize on Speedivac.(It takes about 3 hr.) 6.Resuspend in H2O,and microfuge 5 min.Spotting
Phosphoamino acid analysis:Mark Kamps method
in a screw-cap tube containing 150 µl 5.7 N HCl or enough to submerge your piece of membrane. 3. Incubate in 110°C oven for 60 min. 4. Microfuge sample full speed for at least 1 min. 5. Transfer supernatant to new tube and lyophilize
and neutralizing solution. Tray with neutralizing solution. Whatman #1 filter paper to layer between filters. 80℃ vacuum drying oven (preheated 1 hour). Hybridization buffer (sterile 5x SSPE; 10x filter sterilized Denhardt's solution; sterile 0.1% SDS; use
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