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文献和实验Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
(see below) Flow Cells : Flow cells can be constructed in many different ways. The most common way is to place two strips of double-stick tape on a glass slide ~7-10 mm apart and cover with a 18x18 or 22x22 mm coverslip. This results in a ~12-15 µl flow cell
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
on a glass slide ~7-10 mm apart and cover with a 18x18 or 22x22 mm coverslip. This results in a ~12-15 µl flow cell. Solutions are pipeted on one side and sucked out the other side by capillary action using Whatman #1 filter paper or a Kimwipe. Washes in flow
tube, boil 400 m L/sample or 100 m L/sample of RNA hybridization solution at 100°C for 5 min, for 0.5 mL or 0.2 mL tubes respectively. Cool on ice for at least 5 min. This freshly boiled hybridization solution will be used as the pre-hybridization
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