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文献和实验material can be excised for use in a PCR reaction with a sterile Gilson tip.PCR reaction conditions are as follows:total volume= 50ulfor 5.5 reactions10X buffer 5ul 27.5uls10mM dNTPs 1.25uls 6.875ulsprimer A 2.5uls 13.75ulsprimer B 2.5uls 13.75ulsdH2O 38.75
Tissue Culture - Storage of Cell Lines
, label vials, prepare cryovials, etc.) Freeze only cultures that are healthy and in the mid-log phase of growth (ie., 2-5 x 105 cells/ml). Have your vials already labelled with a waterproof, cryogenic pen. Include cell line
Tissue Culture - Storage of Cell Lines
and resuspend in 10% DMSO/90% FCS at about 1-5 x 106 cells/ml. The freezing medium should be 5℃, not warm. 6.Put 1ml of cell suspension into cryovials and tighten the lid finger tight. Do not over tighten or the vials could explode when thawed. 7.Use the Nunc
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