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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
isothio-cyanate/cesium chloride gradients (Chirgwin et al. 1979 Biochem 18:5294-5299) and can be both expensive and time consuming. Often, however, needs arise where quantitatively smaller amounts of RNA are needed from many different samples, for example
The Cell Environment (including types of culture medium)
free (Prod. No. W3500, water, tissue culture grade). In most cases water prepared by reverse osmosis and resin cartridge purification with a final resistance of 16-18Mx is suitable. Once prepared the media should be filter sterilized before use
Subculture of Suspension Cell Lines
(Prod. No. D2650) Equipment Personal protective equipment (sterile gloves, Laboratory coat, safety visor) Waterbath set to appropriate temperature Microbiological safety cabinet at appropriate containment level CO2 incubator Pre la beled flasks
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