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文献和实验Spotted Microarray Hybridization and Scan
to pellet any particulate material Place the Microarray(s) in a hybridization chamber (TeleChem or GeneMachines)(To prevent arrays from drying, seal array in hybridization chamber with Whatmann paper moistened with 100 ml of H2 O.)
Agrobacterium growth and transformation (农杆菌培养及转化)
. 3. Transfer cells + DNA to pre-chilled (on ice) electroporation cuvettes with either1 or 2 mm gap sizes. Make sure the white cuvette holder from the Bio-Rad machine is also pre-chilled on ice. 4. Take the ice bucket with the cuvettes and cuvette
Agrobacterium growth and transformation
cuvettes with either1 or 2 mm gap sizes. Make sure the white cuvette holder from the Bio-Rad machine is also pre-chilled on ice. 4. Take the ice bucket with the cuvettes and cuvette holder to the gene pulser. For cuvettes with a 2mm gap size, adjust
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