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文献和实验Cryopreservation of Cell Lines
controlled freezer is recommended. Materials Freeze medium (commonly 70% basal medium, 20% FCS, 10% DMSO (Prod. No. D2650 ) or glycerol, check ECACC data sheets for details). 70% ethanol in water (Prod. No. R8382 ) PBS without Ca
Subculture of Semi-Adherent Cell Lines
and the cells in suspension must be subcultured. Materials Media� pre-warmed to 37ºC (refer to the ECACC Cell Line Data Sheet for the correct medium) 70% ethanol in water (Prod. No. R8382 ) PBS without Ca2+ /Mg2+ (Prod. No. D8537
at 70-80% confluency or as advised on ECACC's cell culture data sheet.Do not allow media to go out of date. Shelf life is only 6 weeks at +4oC once glutamine and serum is added.Avoid water baths from becoming dirty by using Sigma Clean (Prod. No. S5525
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