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文献和实验, and allow the cells to grow for 24 hours in the 37degC shaker/incubator at 350 rpm. 2. Remove block from the shaker/incubator and collect the cells by centrifugation at 2500 rpm for 7 minutes. The cells can be stored frozen at -20degC in the block
(the melting temperature of paraffin). The blocks are transferred to pure paraffin in the oven for 1 hour and then into a second pot of melted paraffin for an additional 2--3 hours. During this time the tissue block is completely infiltrated with melted
are transferred to pure paraffin in the oven for 1 hour and then into a second pot of melted paraffin for an additional 2--3 hours. During this time the tissue block is completely infiltrated with melted paraffin. Subsequent to infiltration, the tissue
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