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文献和实验The In Situ PCR:Amplification and Detection in a Cellular Context
which will be retained within the cell [900-1300 bp] virtually impossible. To resolve these dilemma, we designed a set of primer pairs whose individual reaction products were 1] small enough [app 350 bases] for efficient amplification within the constraints of fixed
and the sensor and slides with tissue sections were placed in a rack in a Bios Oven Thermal Cycler. After 25 cycles (92℃ denaturation for two minutes; 42℃ annealing for two minutes; 72℃ extension for 15 minutes), the slides were removed from the bags and washed
. 2. Yu N, Atienza JM, Bernard J, Blanc S, Zhu J, Wang X, Xu X, Abassi YA.(2006).“Real-time monitoring of morphological changes in liv¬ing cells by electronic cell sensor arrays: an approach to study G protein-coupled receptors”. Anal Chem. 78:35-43
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