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文献和实验with a fluorescent dye. Probes are labeled with one of the four fluorescent dyes. The color of a fluorescent dye indicates 4 of 16 possible dinucleotides in the 1st and 2nd position of the probe. A random probe is ligated to a phosphorylated primer annealed
We recommend that you first download and read this page as a PDF file . Using that as your guide, you can then follow the protocol below and view a Quicktime movie demonstrating the key steps. We have also included higher resolution stills
Probe Design for Expression Arrays Using OligoWiz
. This is followed by a detailed step-by-step guide to designing expression arrays with OligoWiz. The scope of this chapter is exclusively on expression arrays. For an in-depth review of the entire field of probe design (including a comparison of different
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