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文献和实验Methods for Chromatographic Removal of Endotoxin
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition
Removal of Counts : NaOH Method
Removal of Counts : NaOH Method Unwrap the membranes and stack them in a pile (Do not dry out the membranes completely). Place them individually into 1L 0.1 N NaOH 0.2 % SDS wash solution for exactly 20 min. Pour
Carbohydrate Gel Electrophoresis
Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of the reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE
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