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文献和实验Embryo Manipulation Pipettes (Flushers etc.)
Transfer Pipettes Glass used for making transfer pipettes : GC120-15 (350 pieces) by Clark Electromedical Instruments. OUTSIDE DIAMETER: 1.2mm INSIDE DIAMETER: 0.69mm LENGTH: 150mm Agent in Australia : SDR Clinical Technology,
PREPARATION OF MICROINJECTION PIPETTES
Using Program 6 (VH) Heat = 915 Pull = 20 Vel = 90 Time = 120 Pressure = 500 Injection pipettes:- Injection pipettes should have an internal diameter only marginally larger than the stem cells (18-20 um) and a sharp point which allows
-11, inner diameter: 2.4 mm) into 65 cm (cut a top at an angle of about 20 degrees) for gel holder. insert it into a glass tube from the bottom, and then a sharp tip of the tubing may exert form the top. Pull out the tip about 2 cm with a plier
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