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文献和实验100°C Heater block 32P-labled probe use 10 - 20 ng DNA/filter, or about 10 million CPM/filter for Southern's Nylon filter DNA previously blotted onto filter (e
RADIOLABELING OF PROBES FOR ELECTROPHORETIC MOBILITY SHIFT ASSAYS
of solution is in the tube. Check all components of the beta shield device to ensure that there is NO contamination. 14.Add 1μl of Lower strand oligonucleotide to the eppendorf tube,and flick gently to mix. Remove the 100℃ block from the heater and place
Radiolabeling of probes for electrophoretic mobility shift assays
that there is NO contamination. 14、 Add 1 ul of Lower strand oligonucleotide to the eppendorf tube, and flick gently to mix. 15、Remove the 100 C block from the heater and place this on your benchtop (NOT in the hood!! ). Place the eppendorf tube in the block and cover
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