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文献和实验消化PC12是个错误观点,其一针对悬浮PC12,胰酶消化的效果不好证实,其二,我养的PC12,性质贴壁,用100%胰酶消化1小时,愣是没动静,细胞甚至不变圆。最后用力拍打才可以,但代价是细胞损伤非常大,不好恢复。其三,ATCC上明确建议用细胞刮勺刮取PC12,目前我改用刮勺,细胞传代后状态要比用胰酶消化的好很多。 4.再者关于1640和DMEM的问题,我个人观点,二者的主要区别在于DMEM含糖量高,神经细胞都喜欢这,另外1640含一些离子,是防止培养的细胞贴壁。1640一开始主要应用于单克
Evaluation of PCB Dechlorination in Sediments
Polychlorinated biphenyls (PCBs) are important environmental contaml- nants. They are widely distributed and have been associated with toxic effects in wildlife, such as deformities and lowered reproductive success (1 ), and with hepatic
Methods for Evaluation of PCB Dechlorination in Sediments
chlorinated congeners formed tend to be more aerobically degradable and less toxic. Remedial action plans for PCB-contaminated sediments should therefore take mto account the extent of dechlorination that has occurred and the potential
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