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文献和实验Immunofluorescence on ultrathin resin sections
General remarks: Ultrathin methacrylate-(e.g.Lowicryl HM20, K4M, Monostep polar and nonpolar or LR-White) or epoxy (Epon/Araldite, Spurr) sections are transferred with a loop on poly-L-lysine coated, round cover slips
), cross-linked to the inert membrane, and carried through 25 to 30 Edman cycles. The sequence of the target protein is entered into the CRP program. This program predicts how many Edman cycles are required to cover 100% of all the serines, threonines
Immunofluorescence on ultrathin resin sections
.Lowicryl HM20, K4M, Monostep polar and nonpolar or LR-White) or epoxy (Epon/Araldite, Spurr) sections are transferred with a loop on poly-L-lysine coated, round cover slips. Residual water is drained with filter paper along the outside of the loop
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