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文献和实验Replication timing by density transfer
), arrest with 200 nM alpha factor (1-1.25 doubling times, until % unbudded cells stops increasing). NOTE: This alpha factor concentration is for bar1 strains. BAR1+ strains require 15x more alpha factor. 3. Filter, wash in Ycomp+alpha factor, resuspend
MgCl2 + 0.5 mls formaldehyde. Incubate 1 hr at 30¡C (or previous temp). Wash the cells two times in the previous buffer (no formaldehyde) and once in the same buffer containing 1.2M sorbitol. Be very gentle with the cells! Resuspend in 0.5 mls
great for us. Most of the procedure is derived from the Boehringer Mannheim manual "The Genius System User's Guide for Filter Hybridization, Version 2.0, catalogue number 101 023, and the product information flyer for disodium 3-(4-methoxyspiro
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