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文献和实验Basic Analysis of NimbleGen ChIP-on-chip Data using Bioconductor/R
Introduction Hybridization of chromatin immuno-precipitation (ChIP) material to tiling arrays at NimbleGen service facilities usually leaves the customer with a set of data files that are of limited use. Most information about the experiment
1. Add appropriate volumes of RNA ( Try 5 and 10 ug RNA plus 600 or 800 pg dig labelled probe. 2. Include 2-3 yeast RNA samples/probe used. 2ul of 5mg/ml stock. 3. Add 20 ul of Soln. A to all of the tubes (F.V.= 30 ul) vortex
1. Add appropriate volumes of RNA ( Try 5 and 10 ug RNA plus 600 or 800 pg dig labelled probe. 2. Include 2-3 yeast RNA samples/probe used. 2ul of 5mg/ml stock. 3. Add 20 ul of Soln. A to all of the tubes (F.V.= 30 ul) vortex and spin
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