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文献和实验Obtaining Embryos: hCG Injection
the needle parallel for injection. The needle should penetrate pretty easily. If it doesn't, then the needle has probably become blunt, and it is worth switching the needle or the frog will get an abrasion. Be patient and careful with trops! X. laevis
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
Sucrose Fractionation of Castor Bean
the gradient. Alternatively, the gradient can be fractionated by puncturing the bottom of the tubes with a needle, and collecting the fractions in 1.5 ml portions by counting the appropriate number of drops that drip from the punctured tube
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