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上海希言科学仪器有限公司
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文献和实验Cell Culture Media and Solutions
165.0 ml fetal bovine serum, heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AG Filter sterilize through a 0.22 � cellulose acetate filter and store
, and, by macropinocytosis, produce large amounts of IL-12 on CD40 ligation, and are potent in presenting soluble Ag and in stimulating allogeneic mixed-leukocyte reactions. To study primary T-cell responses and cytokine production in allergy patients
T-cell dependent antigen specific in situ staining (Pe)
Double staining for Ag and Ki-67, TUNEL or BCL-6. Cut frozen tissue sections (spleen, lymph nodes) in a cryostat at ~ 4µm, on plain glass slides (Adhesive -coated slides are sometimes too hydrophobic, but fine
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